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Beclin 1 Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 3 - Beclin 1 Antibody (Ascites) AM1818a
    Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
  • 1 - Beclin 1 Antibody (Ascites) AM1818a
    Western blot analysis of anti-BECN1 Mab (Cat. #AM1818a) in HepG2, 293, Jurkat and Hela cell line lysates (35μg/lane). BECN1 (arrow) was detected using the Mab ascites (1:2000 dilution).
  • 1 - Beclin 1 Antibody (Ascites) AM1818a
    Western blot analysis of anti-BECN1 Mab (Cat. #AM1818a) in recombinant BECN1 protein. BECN1(arrow) was detected using the ascites Mab (1:2000 dilution).
  • 14 - Beclin 1 Antibody (Ascites) AM1818a
    Breast CA section stained with Autophagy Beclin 1 Antibody (Cat. # AM1818a) at a 1:50 dilution. Data courtesy of Dr. Anita Thyagarajan, Cancer Research Laboratory, Methodist Research Institute, Indianapolis, Indiana.
  • 产品详情
  • 文献引用 : 5
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, IHC-P, E
Primary Accession Q14457
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG3λ
Clone Names 15CT26
Calculated MW 51896 Da
Additional info
Gene ID 8678
Other Names Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, BECN1, GT197
Target/Specificity This Beclin 1 antibody was raised using purified His-tagged recombinant full length human Autophagy BECN1.
Dilution IF~~1:100
WB~~1:50~2000
IHC-P~~1:50~1:200
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeclin 1 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BECN1
Synonyms GT197
Function Plays a central role in autophagy (PubMed:23184933, PubMed:28445460). Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530, PubMed:26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed:25275521). Protects against infection by a neurovirulent strain of Sindbis virus (PubMed:9765397). May play a role in antiviral host defense.
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:O88597, ECO:0000269|PubMed:19713971, ECO:0000269|PubMed:21364619}. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein. Endosome {ECO:0000250|UniProtKB:O88597}. Cytoplasmic vesicle, autophagosome. Note=Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells (By similarity) {ECO:0000250|UniProtKB:O88597, ECO:0000269|PubMed:19050071} Beclin-1-C 37 kDa: Mitochondrion {ECO:0000250|UniProtKB:O88597}
Tissue Location Ubiquitous.
Research Areas
Mir-30d suppresses cell proliferation of colon cancer cells by inhibiting cell autophagy and promoting cell apoptosis.
Author : Zhang R1,Xu J1,Zhao J1,Bai J2.
Tumour Biol. 2017 Jun;39(6):1010428317703984. doi: 10.1177/1010428317703984.
28651493
Echovirus 7 entry into polarized caco-2 intestinal epithelial cells involves core components of the autophagy machinery.
Author : Kim C, Bergelson JM.
J Virol. 2014 Jan;88(1):434-43. doi: 10.1128/JVI.02706-13. Epub 2013 Oct 23.
24155402
Selective subversion of autophagy complexes facilitates completion of the Brucella intracellular cycle.
Author : Starr T, Child R, Wehrly TD, Hansen B, Hwang S, López-Otin C, Virgin HW, Celli J.
Cell Host Microbe. 2012 Jan 19;11(1):33-45. doi: 10.1016/j.chom.2011.12.002.
22264511
Activation of autophagy in mesenchymal stem cells provides tumor stromal support.
Author : Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR.
Carcinogenesis. 2011 Jul;32(7):964-72. doi: 10.1093/carcin/bgr029. Epub 2011 Feb 11.
21317300
Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease.
Author : Ma JF, Huang Y, Chen SD, Halliday G.
Neuropathol Appl Neurobiol. 2010 Jun;36(4):312-9. doi: 10.1111/j.1365-2990.2010.01067.x. Epub 2010 Jan 21.
20102518

BACKGROUND

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).

REFERENCES

References for protein:
1.Age at onset in Huntington's disease is modified by the autophagy pathway: implication of the V471A polymorphism in Atg7. Metzger S, et al. Hum Genet, 2010 Oct. PMID 20697744.
2.Interaction of Beclin 1 with survivin regulates sensitivity of human glioma cells to TRAIL-induced apoptosis. Niu TK, et al. FEBS Lett, 2010 Aug 20. PMID 20638385.
3.Regulation of amyloid precursor protein processing by the Beclin 1 complex. Jaeger PA, et al. PLoS One, 2010 Jun 15. PMID 20559548.
4.Genetic and epigenetic silencing of the beclin 1 gene in sporadic breast tumors. Li Z, et al. BMC Cancer, 2010 Mar 16. PMID 20230646.
5.Over-expression of the Beclin1 gene upregulates chemosensitivity to anti-cancer drugs by enhancing therapy-induced apoptosis in cervix squamous carcinoma CaSki cells. Sun Y, et al. Cancer Lett, 2010 Aug 28. PMID 20207475.
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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