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LC3 Antibody (APG8)

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - LC3 Antibody (APG8) AM1800a
    Anti-MAP1LC3A Antibody at 1:5000 dilution + recombinant protein Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - LC3 Antibody (APG8) AM1800a
    All lanes : Anti-MAP1LC3A Antibody at 1:2000 dilution Lane 1: human brain lysates Lane 2: mouse brain lysates Lane 3: rat brain lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - LC3 Antibody (APG8) AM1800a
    Western blot analysis of anti-LC3 Mab (Cat. #AM1800a) at 8 μg/ml. Lane 1: Y79 (soluble fraction of cell extract); Lane 2: 293 transfected with human LC3 (whole cell extract).
  • 1 - LC3 Antibody (APG8) AM1800a
    Western blot analysis of anti-LC3 Mab (Cat. #AM1800a) Hela cell lysates, which were treated with rapamycin or bafilomycin overnight. Data courtesy of Dr. David Rubinsztein, Cambridge Institute for Medical Research.
  • 产品详情
  • 文献引用 : 17
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession Q9H492, Q9GZQ8
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1 k
Clone Names 166AT1234
Additional info
Other Names Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha, MAP1LC3A
Target/Specificity This LC3 antibody is generated from mouse immunized with a full length recombinant protein of human LC3 (APG8).
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLC3 Antibody (APG8) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice.
Author : Ichikawa N1,2,Alves M1,Pfeiffer S1,Langa E1,Hernández-Santana YE1,Suzuki H2,Prehn JH1,Engel T1,Henshall DC1.
Cell Death Dis. 2017 Jan 12;8(1):e2556. doi: 10.1038/cddis.2016.301.
28079889
Cross-talk between lipid and protein carbonylation in a dynamic cardiomyocyte model of mild nitroxidative stress.
Author : Griesser E1,Vemula V1,Raulien N2,Wagner U2,Reeg S3,Grune T4,Fedorova M5.
Redox Biol. 2016 Dec 28;11:438-455. doi: 10.1016/j.redox.2016.12.028. [Epub ahead of print]
28086193
Tamoxifen Induces Cytotoxic Autophagy in Glioblastoma.
Author : Graham CD1, Kaza N1, Klocke BJ1, Gillespie GY1, Shevde LA1, Carroll SL1, Roth KA2.
J Neuropathol Exp Neurol. 2016 Aug 11. pii: nlw071. [Epub ahead of print]
27516117
Lapatinib induces autophagic cell death and differentiation in acute myeloblastic leukemia.
Author : Chen YJ1, Fang LW2, Su WC3, Hsu WY4, Yang KC4, Huang HL5.
Onco Targets Ther. 2016 Jul 20;9:4453-64. doi: 10.2147/OTT.S105664. eCollection 2016.
27499639
Male meiotic cytokinesis requires ceramide synthase 3-dependent sphingolipids with unique membrane anchors.
Author : Rabionet M1, Bayerle A1, Jennemann R2, Heid H3, Fuchser J4, Marsching C5, Porubsky S6, Bolenz C7, Guillou F8, Gröne HJ2, Gorgas K9, Sa
Hum Mol Genet. 2015 Jun 4. pii: ddv204. [Epub ahead of print]
26045466
GMI, an immunomodulatory protein from Ganoderma microsporum, potentiates cisplatin-induced apoptosis via autophagy in lung cancer cells.
Author : Hsin IL, Ou CC, Wu MF, Jan MS, Hsiao YM, Lin CH, Ko JL.
Mol Pharm. 2015 Mar 26. [Epub ahead of print]
25811903
CD40 ligand exhibits a direct antiviral effect on Herpes Simplex Virus type-1 infection via a PI3K-dependent, autophagy-independent mechanism.
Author : Vlahava VM1, Eliopoulos AG2, Sourvinos G3.
Cell Signal. 2015 Mar 9. pii: S0898-6568(15)00078-9. doi: 10.1016/j.cellsig.2015.03.002. [Epub ahead of print]
25765994
Atorvastatin induces autophagic cell death in prostate cancer cells in vitro.
Author : He Z1, Yuan J2, Qi P3, Zhang L3, Wang Z1.
Mol Med Rep. 2015 Feb 11. doi: 10.3892/mmr.2015.3334. [Epub ahead of print]
25672364
Immunohistochemical study of the autophagy marker microtubule-associated protein 1 light chain 3 in normal and steatotic human livers.
Author : Kashima J1, Shintani-Ishida K, Nakajima M, Maeda H, Unuma K, Uchiyama Y, Yoshida K.
Hepatol Res. 2014 Jul;44(7):779-87. doi: 10.1111/hepr.12183. Epub 2013 Aug 7.
23773367
The effect of RNAi silencing of p62 using an osmotic polysorbitol transporter on autophagy and tumorigenesis in lungs of K-ras(LA1) mice.
Author : Islam MA1, Shin JY2, Yun CH1, Cho CS3, Seo HW2, Chae C2, Cho MH4.
Biomaterials. 2014 Feb;35(5):1584-96. doi: 10.1016/j.biomaterials.2013.11.010. Epub 2013 Nov 22.
24269155
Production of interferon α by human immunodeficiency virus type 1 in human plasmacytoid dendritic cells is dependent on induction of autophagy.
Author : Zhou D1, Kang KH, Spector SA.
J Infect Dis. 2012 Apr 15;205(8):1258-67. doi: 10.1093/infdis/jis187. Epub 2012 Mar 6.
22396599
Induction of autophagy is essential for monocyte-macrophage differentiation.
Author : Zhang Y, Morgan MJ, Chen K, Choksi S, Liu ZG.
Blood. 2012 Mar 22;119(12):2895-905. doi: 10.1182/blood-2011-08-372383. Epub 2012 Jan 5.
22223827
Beclin 1 knockdown inhibits autophagic activation and prevents the secondary neurodegenerative damage in the ipsilateral thalamus following focal cerebral infarction.
Author : Xing S1, Zhang Y, Li J, Zhang J, Li Y, Dang C, Li C, Fan Y, Yu J, Pei Z, Zeng J.
Autophagy. 2012 Jan;8(1):63-76. doi: 10.4161/auto.8.1.18217. Epub 2012 Jan 1.
22108007
Mouse knock-out of IOP1 protein reveals its essential role in mammalian cytosolic iron-sulfur protein biogenesis.
Author : Song D1, Lee FS.
J Biol Chem. 2011 May 6;286(18):15797-805. doi: 10.1074/jbc.M110.201731. Epub 2011 Mar 2.
21367862
Characterization of Puma-dependent and Puma-independent neuronal cell death pathways following prolonged proteasomal inhibition.
Author : Tuffy LP, Concannon CG, D'Orsi B, King MA, Woods I, Huber HJ, Ward MW, Prehn JH.
Mol Cell Biol. 2010 Dec;30(23):5484-501. doi: 10.1128/MCB.00575-10. Epub 2010 Oct 4.
20921277
The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5.
Author : Rouschop KM, van den Beucken T, Dubois L, Niessen H, Bussink J, Savelkouls K, Keulers T, Mujcic H, Landuyt W, Voncken JW, Lambin P, van der Kogel AJ, Koritzinsky M, Wouters BG.
J Clin Invest. 2010 Jan;120(1):127-41. doi: 10.1172/JCI40027. Epub 2009 Dec 14.
20038797
The Rac1/MKK7/JNK pathway signals upregulation of Atg5 and subsequent autophagic cell death in response to oncogenic Ras.
Author : Byun JY, Yoon CH, An S, Park IC, Kang CM, Kim MJ, Lee SJ.
Carcinogenesis. 2009 Nov;30(11):1880-8. doi: 10.1093/carcin/bgp235. Epub 2009 Sep 26.
19783847

BACKGROUND

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

References for protein:
1.Autophagy negatively regulates Wnt signalling by promoting Dishevelled degradation. Gao C, et al. Nat Cell Biol, 2010 Aug. PMID 20639871.
2.The prolyl isomerase Pin1 induces LC-3 expression and mediates tamoxifen resistance in breast cancer. Namgoong GM, et al. J Biol Chem, 2010 Jul 30. PMID 20479004.
3.Protein kinase C inhibits autophagy and phosphorylates LC3. Jiang H, et al. Biochem Biophys Res Commun, 2010 May 14. PMID 20398630.
4. Processing of autophagic protein LC3 by the 20S proteasome. Gao Z, et al. Autophagy, 2010 Jan. PMID 20061800.
5.Defective autophagy associated with LC3 puncta in epothilone-resistant cancer cells. Shen S, et al. Cell Cycle, 2010 Jan 15. PMID 20023420.
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

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