|Application ||WB, IHC-P, IF, E|
|Calculated MW||22743 Da|
|Other Names||Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1, LIN28A, CSDD1, LIN28, ZCCHC1|
|Target/Specificity||LIN28A recombinant protein is used to produce this monoclonal antibody.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LIN28A Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CSDD1, LIN28, ZCCHC1|
|Function||Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.|
|Cellular Location||Cytoplasm. Nucleus, nucleolus. Note=Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.|
|Tissue Location||Expressed in embryonic stem cells (ES cells), placenta and testis.|
Pluripotency factors Lin28 and Oct4 identify a sub-population of stem cell-like cells in ovarian cancer. Peng S, et al. Oncogene, 2010 Apr 8. PMID 20101213.
Lin28-mediated post-transcriptional regulation of Oct4 expression in human embryonic stem cells. Qiu C, et al. Nucleic Acids Res, 2010 Mar. PMID 19966271.
An epigenetic switch involving NF-kappaB, Lin28, Let-7 MicroRNA, and IL6 links inflammation to cell transformation. Iliopoulos D, et al. Cell, 2009 Nov 13. PMID 19878981.
TUT4 in concert with Lin28 suppresses microRNA biogenesis through pre-microRNA uridylation. Heo I, et al. Cell, 2009 Aug 21. PMID 19703396.
A role for Lin28 in primordial germ-cell development and germ-cell malignancy. West JA, et al. Nature, 2009 Aug 13. PMID 19578360.