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SET07 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - SET07 Antibody AM1191a
    Western blot analysis of lysates from 293, 293T cell line (from left to right), using SET07 Antibody(43AT551. 86. 76). 43AT551. 86. 76 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 14 - SET07 Antibody AM1191a
    Formalin-fixed and paraffin-embedded human testis tissue reacted with SET07 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - SET07 Antibody AM1191a
    (TOP)Negative control of hela cells without PE-conjugated goat anti-mouse lgG (whole molecule).PE-conjugated goat anti-mouse lgG emits red fluorescence. ( Bottom)Immunofluorescence analysis of SET07 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB, E
Primary Accession Q9NQR1
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 43AT551.86
Calculated MW 42890 Da
Additional info
Gene ID 387893
Other Names N-lysine methyltransferase SETD8, 211-, H4-K20-HMTase SETD8, Histone-lysine N-methyltransferase SETD8, Lysine N-methyltransferase 5A, PR/SET domain-containing protein 07, PR-Set7, PR/SET07, SET domain-containing protein 8, SETD8, KMT5A, PRSET7, SET07, SET8
Target/Specificity This SET07 antibody was raised using purified recombinant GST fusion protein encoding the N-terminal region of human SET07.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSET07 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KMT5A (HGNC:29489)
Function Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. Specifically monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contributing to the maintenance of proper higher-order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53/TP53 at 'Lys-382', leading to repress p53/TP53-target genes. Plays a negative role in TGF-beta response regulation and a positive role in cell migration.
Cellular Location Nucleus. Chromosome. Note=Specifically localizes to mitotic chromosomes. Colocalized with SIRT2 at mitotic foci. Associates with chromosomes during mitosis; association is increased in a H(2)O(2)-induced oxidative stress- dependent manner. Associates with silent chromatin on euchromatic arms. Not associated with constitutive heterochromatin
Research Areas

REFERENCES

An miR-502-binding site single-nucleotide polymorphism in the 3'-untranslated region of the SET8 gene is associated with early age of breast cancer onset. Song F, et al. Clin Cancer Res, 2009 Oct 1. PMID 19789321.
Product specificity and mechanism of protein lysine methyltransferases: insights from the histone lysine methyltransferase SET8. Zhang X, et al. Biochemistry, 2008 Jun 24. PMID 18512960.
Catalytic function of the PR-Set7 histone H4 lysine 20 monomethyltransferase is essential for mitotic entry and genomic stability. Houston SI, et al. J Biol Chem, 2008 Jul 11. PMID 18480059.
PR-Set7 establishes a repressive trans-tail histone code that regulates differentiation. Sims JK, et al. Mol Cell Biol, 2008 Jul. PMID 18474616.
SET8 plays a role in controlling G1/S transition by blocking lysine acetylation in histone through binding to H4 N-terminal tail. Yin Y, et al. Cell Cycle, 2008 May 15. PMID 18418072.

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