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TBB5 Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - TBB5 Antibody AM1031A
    Western blot analysis of lysates from 293, CEM, Hela, Y79, rat PC-12 cell line, mouse brain tissue lysate(from left to right), using TBB5 Antibody(Cat. #AM1031a). AM1031a was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
  • 14 - TBB5 Antibody AM1031A
    Immunohistochemical analysis of paraffin-embedded H.kidney section using TBB5 Antibody(Cat#AM1031a). AM1031a was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - TBB5 Antibody AM1031A
    Immunofluorescent analysis of A549 cells, using TBB5 Antibody (Cat. #AM1031a). AM1031a was diluted at 1:25 dilution. Dylight Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red).
  • 14 - TBB5 Antibody AM1031A
    Formalin-fixed and paraffin-embedded human testis reacted with TBB5 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 产品详情
  • 文献引用 : 5
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, IF, E
Primary Accession P07437
Other Accession P09244, Q91575, P69897, Q767L7, P99024, P69893, Q2KJD0
Reactivity Human, Mouse, Rat
Predicted Bovine, Chicken, Hamster, Pig, Rat, Xenopus
Host Mouse
Clonality Monoclonal
Isotype IgG2b,Igκ
Clone Names 87CT59.3.7
Calculated MW 49671 Da
Additional info
Gene ID 203068
Other Names Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5
Target/Specificity TBB5 recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:1000
IHC-P~~1:10~50
IF~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTBB5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB
Synonyms TUBB5
Function Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
Cellular Location Cytoplasm, cytoskeleton.
Tissue Location Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
Research Areas
TLR4-mediated activation of the ERK pathway following UVA irradiation contributes to increased cytokine and MMP expression in senescent human dermal fibroblasts.
Author : Seo SW1,Park SK1,Oh SJ1,Shin OS1.
PLoS One. 2018 Aug 17;13(8):e0202323. doi: 10.1371/journal.pone.0202323. eCollection 2018.
30118525
Wnt pathway is involved in 5-FU drug resistance of colorectal cancer cells.
Author : He L1,Zhu H1,Zhou S1,Wu T1,Wu H1,Yang H1,Mao H1,SekharKathera C1,Janardhan A1,Edick AM2,Zhang A3,Hu Z1,Pan F1,Guo Z4.
Exp Mol Med. 2018 Aug 14;50(8):101. doi: 10.1038/s12276-018-0128-8.
30111797
Obesity-induced overexpression of miRNA-24 regulates cholesterol uptake and lipid metabolism by targeting SR-B1.
Author : Wang M1,Li L1,Liu R1,Song Y1,Zhang X1,Niu W1,Kumar AK1,Guo Z1,Hu Z2.
Gene. 2018 May 19. pii: S0378-1119(18)30569-9. doi: 10.1016/j.gene.2018.05.072. [Epub ahead of print]
29787826
OGG1 regulates the level of symmetric dimethylation of histone H4 arginine-3 by interacting with PRMT5.
Author : Zhou X1,Wang W2,Du C1,Yan F1,Yang S1,He K1,Wang H1,Zhao A3.
Mol Cell Probes. 2018 Feb 2. pii: S0890-8508(18)30030-6. doi: 10.1016/j.mcp.2018.01.002. [Epub ahead of print]
29409673
OGG1 is essential in oxidative stress induced DNA demethylation.
Author : Zhou X1, Zhuang Z2, Wang W1, He L1, Wu H1, Cao Y1, Pan F1, Zhao J1, Hu Z1, Sekhar C1, Guo Z3.
Cell Signal. 2016 May 29. pii: S0898-6568(16)30128-0. doi: 10.1016/j.cellsig.2016.05.021. [Epub ahead of print]
27251462

REFERENCES

An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014. High-density SNP screening of the major histocompatibility complex in systemic lupus erythematosus demonstrates strong evidence for independent susceptibility regions. Barcellos LF, et al. PLoS Genet, 2009 Oct. PMID 19851445. Proteome analysis of schizophrenia patients Wernicke's area reveals an energy metabolism dysregulation. Martins-de-Souza D, et al. BMC Psychiatry, 2009 Apr 30. PMID 19405953. Identification of interaction partners for individual SH3 domains of Fas ligand associated members of the PCH protein family in T lymphocytes. Linkermann A, et al. Biochim Biophys Acta, 2009 Feb. PMID 19041431. Alterations in oligodendrocyte proteins, calcium homeostasis and new potential markers in schizophrenia anterior temporal lobe are revealed by shotgun proteome analysis. Martins-de-Souza D, et al. J Neural Transm, 2009 Mar. PMID 19034380.

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