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>   首页   >   产品   >   一抗   >   精选抗体   >   内参抗体   >   Beta-actin Antibody   

Beta-actin Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
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  • 1 - Beta-actin Antibody AM1021B
    Western blot analysis of lysates from HepG2, HL-60 cell line (from left to right), using Beta-actin Antibody(Cat. #AM1021b). AM1021b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
  • 14 - Beta-actin Antibody AM1021B
    Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#AM1021b). AM1021b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - Beta-actin Antibody AM1021B
    Western blot analysis of lysates from HepG2, HL-60, mouse NIH/3T3 cell line, mouse cerebellum and rat stomach tissue lysate, CHO, COS-7 cell line lysate(from left to right), using Beta-actin Antibody(Cat. #AM1021b). AM1021b was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
  • 14 - Beta-actin Antibody AM1021B
    Immunohistochemical analysis of paraffin-embedded H. spleen section using Beta-actin Antibody(Cat#AM1021b). AM1021b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 产品详情
  • 文献引用 : 54
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P60709
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG
Clone Names 8H10D10
Additional info
Gene ID 60
Other Names Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Target/Specificity ACTB recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBeta-actin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ACTB
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Research Areas
TRPV4 Channels Contribute to Renal Myogenic Autoregulation in Neonatal Pigs.
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Am J Physiol Renal Physiol. 2017 Aug 2:ajprenal.00300.2017. doi: 10.1152/ajprenal.00300.2017. [Epub ahead of print]
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Zinc Depletion by TPEN Induces Apoptosis in Human Acute Promyelocytic NB4 Cells.
Author : Zhu B1,Wang J1,Zhou F1,Liu Y1,Lai Y1,Wang J1,Chen X1,Chen D1,Luo L1,Hua ZC1,2.
Cell Physiol Biochem. 2017 Jul 27;42(5):1822-1836. doi: 10.1159/000479539. [Epub ahead of print]
28750402
PML-RARα stabilized by zinc in human acute promyelocytic leukemia NB4 cells.
Author : Zhu B1,Wang JY1,Zhou JJ1,Zhou F1,Cheng W1,Liu YT1,Wang J1,Chen X1,Chen DH1,Luo L2,Hua ZC3.
J Inorg Biochem. 2017 Jul 19;175:92-100. doi: 10.1016/j.jinorgbio.2017.07.007. [Epub ahead of print]
28743050
Schlafen 14 (SLFN14) is a novel antiviral factor involved in the control of viral replication.
Author : Seong RK1,Seo SW1,Kim JA1,Fletcher SJ2,Morgan NV2,Kumar M3,Choi YK4,Shin OS5.
Immunobiology. 2017 Jul 11. pii: S0171-2985(17)30115-8. doi: 10.1016/j.imbio.2017.07.002. [Epub ahead of print]
28734654
Protective Effects of Geniposide on Hepatic Ischemia/Reperfusion Injury.
Author : Rong YP1,Huang HT2,Liu JS2,Wei L2.
Transplant Proc. 2017 Jul - Aug;49(6):1455-1460. doi: 10.1016/j.transproceed.2017.02.063.
28736023
Overexpression of ubiquitin specific peptidase 14 predicts unfavorable prognosis in esophageal squamous cell carcinoma.
Author : Zhang B1,2,Li M1,Huang P3,Guan XY1,4,Zhu YH1.
Thorac Cancer. 2017 May 16. doi: 10.1111/1759-7714.12453. [Epub ahead of print]
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SBI0206965, a novel inhibitor of Ulk1, suppresses non-small cell lung cancer cell growth by modulating both autophagy and apoptosis pathways.
Author : Tang F1,Hu P2,Yang Z3,Xue C3,Gong J1,Sun S1,Shi L1,Zhang S1,Li Z1,Yang C1,Zhang J1,Xie C1.
Oncol Rep. 2017 May 10. doi: 10.3892/or.2017.5635. [Epub ahead of print]
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UVRAG Deficiency Exacerbates Doxorubicin-Induced Cardiotoxicity.
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Metformin Alleviates Aging Cellular Phenotypes in Hutchinson-Gilford Progeria Syndrome Dermal Fibroblasts.
Author : Park SK1,Shin OS1.
Exp Dermatol. 2017 Feb 13. doi: 10.1111/exd.13323. [Epub ahead of print]
28192606
ACY-1215 accelerates vemurafenib induced cell death of BRAF-mutant melanoma cells via induction of ER stress and inhibition of ERK activation.
Author : Peng U1,Wang Z2,Pei S1,Ou Y1,Hu P2,Liu W2,Song J1.
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28035401
Redistribution of adrenomedullary nicotinic acetylcholine receptor subunits and the effect on circulating epinephrine levels in a murine model of acute asthma.
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Int J Mol Med. 2016 Dec 23. doi: 10.3892/ijmm.2016.2836. [Epub ahead of print]
28035367
STAT3 signaling drives EZH2 transcriptional activation and mediates poor prognosis in gastric cancer.
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Mol Cancer. 2016 Dec 9;15(1):79.
27938379
Anti-Inflammatory Effects of Chloranthalactone B in LPS-Stimulated RAW264.7 Cells.
Author : Li X1,Shen J2,Jiang Y3,4,Shen T5,6,You L7,8,Sun X9,Xu X10,Hu W11,12,Wu H13,Wang G14.
Int J Mol Sci. 2016 Nov 22;17(11). pii: E1938.
27879664
CUG-binding protein 1 regulates HSC activation and liver fibrogenesis.
Author : Wu X1,Wu X1,Ma Y1,Shao F1,Tan Y1,Tan T1,Gu L1,Zhou Y1,Sun B2,Sun Y1,Wu X1,Xu Q1.
Nat Commun. 2016 Nov 17;7:13498. doi: 10.1038/ncomms13498.
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STARD13 promotes hepatocellular carcinoma apoptosis by acting as a ceRNA for Fas.
Author : Zhang H1,Wang F1,Hu Y2.
Biotechnol Lett. 2016 Nov 14. [Epub ahead of print]
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Inhibitory effects of silibinin on proliferation and lung metastasis of human high metastasis cell line of salivary gland adenoid cystic carcinoma via autophagy induction.
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Onco Targets Ther. 2016 Oct 26;9:6609-6618. eCollection 2016.
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Tumor-targeted delivery of a C-terminally truncated FADD (N-FADD) significantly suppresses the B16F10 melanoma via enhancing apoptosis.
Author : Yang YW1, Zhang CM1, Huang XJ1, Zhang XX1, Zhang LK1, Li JH1,2, Hua ZC1,2.
Sci Rep. 2016 Oct 21;6:34178. doi: 10.1038/srep34178.
27767039
Deletion of autophagy-related gene 7 in dopaminergic neurons prevents their loss induced by MPTP.
Author : Niu XY1, Huang HJ1, Zhang JB1, Zhang C2, Chen WG1, Sun CY3, Ding YQ4, Liao M5.
Neuroscience. 2016 Sep 27. pii: S0306-4522(16)30484-5. doi: 10.1016/j.neuroscience.2016.09.037. [Epub ahead of print]
27693472
Huaier aqueous extract inhibits proliferation and metastasis of tuberous sclerosis complex cell models through downregulation of JAK2/STAT3 and MAPK signaling pathways.
Author : Yang A1,Fan H2,Zhao Y1,Zha X3,Zhang H4,Hu Z1,Tu P1.
Oncol Rep. 2016 Sep;36(3):1491-8. doi: 10.3892/or.2016.4969. Epub 2016 Jul 25.
27461043
Ethyl Pyruvate Combats Human Leukemia Cells but Spares Normal Blood Cells.
Author : Birkenmeier G1, Hemdan NY1, Kurz S1, Bigl M1, Pieroh P2,3, Debebe T4,5, Buchold M1, Thieme R6, Wichmann G7, Dehghani F3.
PLoS One. 2016 Aug 31;11(8):e0161571. doi: 10.1371/journal.pone.0161571. eCollection 2016.
27579985
Insulin-like growth factor 1 promotes the proliferation and committed differentiation of human dental pulp stem cells through MAPK pathways.
Author : Lv T1, Wu Y1, Mu C1, Liu G2, Yan M3, Xu X1, Wu H1, Du J1, Yu J4, Mu J5.
Arch Oral Biol. 2016 Aug 20;72:116-123. doi: 10.1016/j.archoralbio.2016.08.011. [Epub ahead of print]
27573439
Huaier restrains proliferative and invasive potential of human hepatoma SKHEP-1 cells partially through decreased Lamin B1 and elevated NOV.
Author : Hu Z1, Yang A1,2, Su G1,2, Zhao Y1, Wang Y3, Chai X1, Tu P1.
Sci Rep. 2016 Aug 9;6:31298. doi: 10.1038/srep31298.
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Inhibition of IL-6 trans-signaling in the brain increases sociability in the BTBR mouse model of autism.
Author : Wei H1, Ma Y2, Liu J2, Ding C2, Jin G2, Wang Y2, Hu F3, Yu L4.
Biochim Biophys Acta. 2016 Jul 23. pii: S0925-4439(16)30181-8. doi: 10.1016/j.bbadis.2016.07.013. [Epub ahead of print]
27460706
Splicing mutation in Sbf1 causes nonsyndromic male infertility in the rat.
Author : Liska F1, Chylikova B2, Janku M3, Seda O4, Vernerova Z5, Pravenec M6, Kren V7.
Reproduction. 2016 Jun 22. pii: REP-16-0042. [Epub ahead of print]
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12b-hydroxy-des-D-garcigerin A enhances glucose metabolism in insulin-resistant HepG2 cells via the IRS-1/PI3-K/Akt cell signaling pathway.
Author : Chen Y1, Wang S2, Tian ST3, Hu X2, Xu J2, Yang GZ2, Wang CY3.
J Asian Nat Prod Res. 2016 Jun 10:1-10. [Epub ahead of print]
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HMGB1 knockdown effectively inhibits the progression of rectal cancer by suppressing HMGB1 expression and promoting apoptosis of rectal cancer cells.
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Mol Med Rep. 2016 May 24. doi: 10.3892/mmr.2016.5340. [Epub ahead of print]
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HDAC6 promotes cell proliferation and confers resistance to gefitinib in lung adenocarcinoma.
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Oncol Rep. 2016 May 16. doi: 10.3892/or.2016.4811. [Epub ahead of print]
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Inhibition of hepatitis B virus replication by a dNTPase-dependent function of the host restriction factor SAMHD1.
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Virology. 2016 May 11;495:71-78. doi: 10.1016/j.virol.2016.05.001. [Epub ahead of print]
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Huaier aqueous extract sensitizes cells to rapamycin and cisplatin through activating mTOR signaling.
Author : Hu Z1, Yang A2, Fan H3, Wang Y4, Zhao Y1, Zha X5, Zhang H6, Tu P7.
J Ethnopharmacol. 2016 Apr 1. pii: S0378-8741(16)30187-8. doi: 10.1016/j.jep.2016.03.069. [Epub ahead of print]
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Abnormal expression of key genes and proteins in the canonical Wnt/β-catenin pathway of articular cartilage in a rat model of exercise-induced osteoarthritis.
Author : Liu SS1,Zhou P1,Zhang Y2.
Mol Med Rep. 2016 Mar;13(3):1999-2006. doi: 10.3892/mmr.2016.4798. Epub 2016 Jan 19.
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The anti-malaria drug artesunate inhibits cigarette smoke and ovalbumin concurrent exposure-induced airway inflammation and might reverse glucocorticoid insensitivity.
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Int Immunopharmacol. 2015 Nov 13. pii: S1567-5769(15)30185-5. doi: 10.1016/j.intimp.2015.11.016. [Epub ahead of print]
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Proteomic Analysis of Cortical Brain Tissue from the BTBR Mouse Model of Autism: Evidence for Changes in Stop and Myelin-Related Proteins.
Author : Wei H1, Ma Y2, Liu J2, Ding C2, Hu F3, Yu L4.
Neuroscience. 2015 Nov 9. pii: S0306-4522(15)00992-6. doi: 10.1016/j.neuroscience.2015.11.003. [Epub ahead of print]
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The association of HLA-G and immune markers in recurrent miscarriages.
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J Matern Fetal Neonatal Med. 2015 Oct 29:1-17. [Epub ahead of print]
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Ancestral TCDD exposure promotes epigenetic transgenerational inheritance of imprinted gene Igf2: Methylation status and DNMTs.
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Toxicol Appl Pharmacol. 2015 Oct 8. pii: S0041-008X(15)30099-5. doi: 10.1016/j.taap.2015.09.024. [Epub ahead of print]
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Int J Clin Exp Pathol. 2015 Jun 1;8(6):6334-44. eCollection 2015.
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20843777

BACKGROUND

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

REFERENCES

Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.

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