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GAPDH Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - GAPDH Antibody AM1020b
    All lanes : Anti-GAPDH Antibody at 1:1000 dilution Lane 1: A431 whole cell lysates Lane 2: C6 whole cell lysates Lane 3: Hela whole cell lysates Lane 4: HUVEC whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GAPDH Antibody AM1020b
    All lanes : Anti-GAPDH Antibody at 1:8000 dilution Lane 1: Hela whole cell lysates Lane 2: A549 whole cell lysates Lane 3: COS-7 whole cell lysates Lane 4: mouse brain lysates Lane 5: C6 whole cell lysates Lane 6: NIH/3T3 whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - GAPDH Antibody AM1020b
    Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 3 - GAPDH Antibody AM1020b
    Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
  • 14 - GAPDH Antibody AM1020b
    Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody(Cat#AM1020b). AM1020b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - GAPDH Antibody AM1020b
    Western blot analysis of anti-GAPDH Monoclonal Antibody (Cat. #AM1020b) in CEM cell line lysates (35μg/lane). GAPDH(arrow) was detected using the purified Mab.
  • 产品详情
  • 文献引用 : 14
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession P04406
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 1A10A10
Additional info
Gene ID 2597
Other Names Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, 2699-, GAPDH, GAPD
Target/Specificity GAPDH recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:2000~10000
IHC-P~~1:25
IF~~1:25
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGAPDH Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GAPDH
Synonyms GAPD
Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D- glyceroyl phosphate. Component of the GAIT (gamma interferon- activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Cellular Location Cytoplasm, cytosol. Nucleus. Cytoplasm, perinuclear region. Membrane. Cytoplasm, cytoskeleton. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Research Areas
l-Rhamnosylation of wall teichoic acids promotes efficient surface association of Listeria monocytogenes virulence factors InlB and Ami through interaction with GW domains.
Author : Carvalho F1,2,Sousa S1,2,Cabanes D1,2.
Environ Microbiol. 2018 Jul 8. doi: 10.1111/1462-2920.14351. [Epub ahead of print]
29984543
DFMG attenuates the activation of macrophages induced by co‑culture with LPC‑injured HUVE‑12 cells via the TLR4/MyD88/NF‑κB signaling pathway.
Author : Cong L1,Yang S2,Zhang Y1,Cao J1,Fu X1.
Int J Mol Med. 2018 May;41(5):2619-2628. doi: 10.3892/ijmm.2018.3511. Epub 2018 Feb 23.
29484368
Peptide SS-31 upregulates frataxin expression and improves the quality of mitochondria: implications in the treatment of Friedreich ataxia.
Author : Zhao H1,Li H1,Hao S1,2,Chen J1,Wu J1,Song C1,Zhang M3,Qiao T3,Li K4.
Sci Rep. 2017 Aug 29;7(1):9840. doi: 10.1038/s41598-017-10320-2.
28852135
Expression and prognostic significance of MYL9 in esophageal squamous cell carcinoma.
Author : Wang JH1,Zhang L2,3,4,5,Huang ST2,3,4,Xu J3,4,Zhou Y2,3,4,Yu XJ3,4,Luo RZ6,Wen ZS7,Jia WH3,4,Zheng M2,3,4.
PLoS One. 2017 Apr 7;12(4):e0175280. doi: 10.1371/journal.pone.0175280. eCollection 2017.
28388691
The Role of Annexin A4 in Triple-Negative Breast Cancer Progression and Its Clinical Application.
Author : Zhang ZG1, Chen JN1, Wang YD1, Gao JT1, Jin Y2.
Ann Clin Lab Sci. 2016 Sep;46(5):515-21.
27650619
Use of rhenium-188 for in vivo imaging and treatment of human cervical cancer cells transfected with lentivirus expressing sodium iodide symporter.
Author : Zhang M1, Shi S1, Guo R1, Miao Y1, Li B1.
Oncol Rep. 2016 Aug 24. doi: 10.3892/or.2016.5034. [Epub ahead of print]
27573304
Silencing DNA methyltransferase 1 (DNMT1) inhibits proliferation, metastasis and invasion in ESCC by suppressing methylation of RASSF1A and DAPK.
Author : Bai J1,Zhang X2,Hu K3,Liu B1,Wang H3,Li A3,Lin F3,Zhang L1,Sun X3,Du Z1,4,Song J1,4.
Oncotarget. 2016 Jul 12;7(28):44129-44141. doi: 10.18632/oncotarget.9866.
27286455
Pulsatile delivery of a leucine supplement during long-term continuous enteral feeding enhances lean growth in term neonatal pigs.
Author : Boutry C1, El-Kadi SW1, Suryawan A1, Steinhoff-Wagner J1, Stoll B1, Orellana RA1, Nguyen HV1, Kimball SR2, Fiorotto ML1, Davis TA3.
Am J Physiol Endocrinol Metab. 2016 Feb 16:ajpendo.00479.2015. doi: 10.1152/ajpendo.00479.2015. [Epub ahead of print]
26884386
Hepatocellular Carcinoma Cells Induce Regulatory T Cells and Lead to Poor Prognosis via Production of Transforming Growth Factor-β1.
Author : Wang Y1, Liu T, Tang W, Deng B, Chen Y, Zhu J, Shen X.
Cell Physiol Biochem. 2016 Jan 25;38(1):306-318. [Epub ahead of print]
26799063
Upregulation of microRNA-96 and its oncogenic functions by targeting CDKN1A in bladder cancer.
Author : Wu Z1, Liu K2, Wang Y2, Xu Z2, Meng J2, Gu S2.
Cancer Cell Int. 2015 Nov 14;15:107.
26582573
SC06, a novel small molecule compound, displays preclinical activity against multiple myeloma by disrupting the mTOR signaling pathway.
Author : Han K1, Xu X1, Xu Z2, Chen G1, Zeng Y1, Zhang Z1, Cao B1, Kong Y2, Tang X3, Mao X1,4.
Sci Rep. 2015 Sep 2;5:12809. doi: 10.1038/srep12809.
26329846
Molecular In Vivo Imaging Using a Noninvasive Cardiac-Specific MLC-2v Promoter Driven Dual-Gene Recombinant Lentivirus Monitoring System.
Author : Zhang M1, Wang L1, Guo R1, Liang S2, Jiang X1, Zhang M1, Li B1.
PLoS One. 2015 Jul 31;10(7):e0133952. doi: 10.1371/journal.pone.0133952. eCollection 2015.
26230833
Cyclin O regulates germinal vesicle breakdown in mouse oocytes.
Author : Ma JY1, Ou-Yang YC, Luo YB, Wang ZB, Hou Y, Han ZM, Liu Z, Schatten H, Sun QY.
Biol Reprod. 2013 May 2;88(5):110. doi: 10.1095/biolreprod.112.103374. Print 2013 May.
23515676
Nutrition deficiency promotes apoptosis of cartilage endplate stem cells in a caspase-independent manner partially through upregulating BNIP3.
Author : He Z1,Pu L1,Yuan C1,Jia M1,Wang J2.
Acta Biochim Biophys Sin (Shanghai). 2016 Nov 17. [Epub ahead of print]
27864279

BACKGROUND

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

REFERENCES

Inhibition of glyceraldehyde-3-phosphate dehydrogenase activity by antibodies present in the cerebrospinal fluid of patients with multiple sclerosis. Kölln J, et al. J Immunol, 2010 Aug 1. PMID 20610654. Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 May 14. PMID 20471030. Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of melanoma antigens using a Serological Proteome Approach (SERPA). Suzuki A, et al. Cancer Genomics Proteomics, 2010 Jan-Feb. PMID 20181627. siah-1 Protein is necessary for high glucose-induced glyceraldehyde-3-phosphate dehydrogenase nuclear accumulation and cell death in Muller cells. Yego EC, et al. J Biol Chem, 2010 Jan 29. PMID 19940145.

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