|Application ||WB, IHC-P, IF, E|
|Calculated MW||50327 Da|
|Other Names||Tubulin beta-1 chain, TUBB1|
|Target/Specificity||This monoclonal antibody is generated from mice immunized with a recombinant protein human TBB1.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TBB1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).|
|Cellular Location||Cytoplasm, cytoskeleton|
|Tissue Location||Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins|
This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.
Study of 18 functional hemostatic polymorphisms in mucocutaneous bleeding disorders. Antón AI, et al. Ann Hematol, 2010 Nov. PMID 20532885.
Natural product derivative Bis(4-fluorobenzyl)trisulfide inhibits tumor growth by modification of beta-tubulin at Cys 12 and suppression of microtubule dynamics. Xu W, et al. Mol Cancer Ther, 2009 Dec. PMID 19996274.
Genotype-phenotype relationship for six common polymorphisms in genes affecting platelet function from 286 healthy subjects and 160 patients with mucocutaneous bleeding of unknown cause. Martínez C, et al. Br J Haematol, 2009 Jun. PMID 19388931.
TUBB1 Q43P polymorphism does not protect against acute coronary syndrome and premature myocardial infarction. Navarro-Núñez L, et al. Thromb Haemost, 2008 Dec. PMID 19132255.
Roles of beta-tubulin residues Ala428 and Thr429 in microtubule formation in vivo. Joe PA, et al. J Biol Chem, 2009 Feb 13. PMID 19074767.