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>   首页   >   产品   >   一抗   >   精选抗体   >   标签抗体   >   GST Antibody   

GST Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - GST Antibody AM1011b
    Western blot analysis of anti-GST Mab in recombinant GST protein. GST(arrow) was detected using the purified Mab .
  • 1 - GST Antibody AM1011b
    Western blot analysis of anti-GST Mab in recombinant GST protein. GST(arrow) was detected using the purified Mab .
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Host Mouse
Clonality Monoclonal
Isotype IgG1 k
Clone Names 21CT54.13.1 / 21CT54.13.7
Calculated MW 26000 Da
Additional info
Other Names Glutathione S-transferase
Target/Specificity Purified recombinant GST fusion protein was used to produced this monoclonal antibody.
Dilution WB~~1:100~500
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGST Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

BACKGROUND

Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.

REFERENCES

Smith, D.B. and Johnson, K.S., (1988). Gene 67, 31. Parker, M.W. et al., (1990) J. Mol. Biol. 213, 221. Toye, B. et al., (1990) Infect. Immun. 58, 3909. Guan, K.L. and Dixon, J.E. (1991) Anal. Biochem. 192, 262

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