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HIS Tag Antibody

Mouse Monoclonal Antibody (Mab)

     
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  • 1 - HIS Tag Antibody AM1010A
    Anti-HIS Tag at 1:8000 dilution + 12-tag lysate Lysates/proteins at 20ng per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HIS Tag Antibody AM1010A
    Anti-HIS Tag at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - HIS Tag Antibody AM1010A
    Western blot analysis of lysates from 12tag protein , This demonstrates the His tagged antibody detected the His tagged protein (arrow).
  • 产品详情
  • 文献引用 : 44
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 6AT18
Calculated MW Da Da
Additional info
Target/Specificity Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.
Dilution WB~~1:1,000
Format Monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIS Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Universal Biofactor-Releasing Scaffold Enabling in Vivo Reloading.
Author : Carasso LS,Benhar I,Dvir T.
Nano Lett. 2019 Mar 5. doi: 10.1021/acs.nanolett.8b04927. [Epub ahead of print]
30817160
Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway.
Author : Miller BK1,Hughes R2,Ligon LS1,Rigel NW1,Malik S1,Anjuwon-Foster BR1,Sacchettini JC2,Braunstein M1.
Elife. 2019 Jan 3;8. pii: e40063. doi: 10.7554/eLife.40063.
30604681
Mass spectrometry-based proteomic analysis reveals the interacting partners of lipin1.
Author : Wang J1,Wang S1,Yan C1,Deng Y2,Huang Z2,Shi P1.
IUBMB Life. 2018 Aug 9. doi: 10.1002/iub.1873. [Epub ahead of print]
30092116
VirB8 homolog TraE from plasmid pKM101 forms a hexameric ring structure and interacts with the VirB6 homolog TraD.
Author : Casu B1,Mary C1,Sverzhinsky A1,Fouillen A1,2,Nanci A1,2,Baron C3.
Proc Natl Acad Sci U S A. 2018 May 21. pii: 201802501. doi: 10.1073/pnas.1802501115. [Epub ahead of print]
29784815
hnRNP R and its main interactor, the noncoding RNA 7SK, coregulate the axonal transcriptome of motoneurons.
Author : Briese M1,Saal-Bauernschubert L1,Ji C1,Moradi M1,Ghanawi H1,Uhl M2,3,Appenzeller S4,5,Backofen R2,3,Sendtner M6.
Proc Natl Acad Sci U S A. 2018 Mar 5. pii: 201721670. doi: 10.1073/pnas.1721670115. [Epub ahead of print]
29507242
Thiol Starvation induces redox-mediated dysregulation of Escherichia coli biofilm components.
Author : Hufnagel DA1,Price JE1,Stephenson RE1,Kelley J1,Benoit MF1,Chapman MR2.
J Bacteriol. 2017 Oct 16. pii: JB.00389-17. doi: 10.1128/JB.00389-17. [Epub ahead of print]
29038256
Metabolically-derived lysine acylations and neighboring modifications tune BET bromodomain binding to histone H4.
Author : Olp M,Zhu N,Smith B.
Biochemistry. 2017 Sep 25. doi: 10.1021/acs.biochem.7b00595. [Epub ahead of print]
28945351
Suppressing ABA uridine diphosphate glucosyltransferase (SlUGT75C1) alters fruit ripening and the stress response in tomato.
Author : Sun Y1,Ji K1,Liang B1,Du Y1,Jiang L1,Wang J1,Kai W1,Zhang Y1,Zhai X1,Chen P1,Wang H1,Leng P1.
Plant J. 2017 May 8. doi: 10.1111/tpj.13588. [Epub ahead of print]
28482127
A chemical genetic screen uncovers a small molecule enhancer of the N-acylethanolamine degrading enzyme, fatty acid amide hydrolase, in Arabidopsis.
Author : Khan BR1,Faure L2,3,Chapman KD2,Blancaflor EB1.
Sci Rep. 2017 Jan 23;7:41121. doi: 10.1038/srep41121.
28112243
A simple strategy for extracellular production of CotA laccase in Escherichia coli and decolorization of simulated textile effluent by recombinant laccase.
Author : Wang TN1, Zhao M2.
Appl Microbiol Biotechnol. 2016 Oct 13. [Epub ahead of print]
27738721
Screening and molecular evolution of a single chain variable fragment antibody (scFv) against Citreoviridin toxin.
Author : Wang R, Gu X, Zhuang Z, Zhong Y, Yang H, Wang S.
J Agric Food Chem. 2016 Sep 13. [Epub ahead of print]
27622814
γ-aminobutyric acid type A (GABAA) receptor subunits play a direct structural role in synaptic contact formation via their N-terminal extracellular domains.
Author : Brown LE1, Nicholson MW2, Arama JE1, Mercer A1, Thomson AM1, Jovanovic JN3.
J Biol Chem. 2016 Apr 20. pii: jbc.M116.714790. [Epub ahead of print]
27129275
Early doors (Edo) mutant mouse reveals the importance of period 2 (PER2) PAS domain structure for circadian pacemaking.
Author : Militi S1, Maywood ES2, Sandate CR3, Chesham JE2, Barnard AR1, Parsons MJ1, Vibert JL1, Joynson GM1, Partch CL3, Hastings MH2, Nolan PM4 Proc Natl Acad Sci U S A. 2016 Feb 22. pii: 201517549. [Epub ahead of print]
26903623
Construction of pTM series plasmids for gene expression in Brucella species.
Author : Tian M1, Qu J1, Bao Y1, Gao J1, Liu J1, Wang S1, Sun Y1, Ding C1, Yu S2.
J Microbiol Methods. 2016 Feb 3. pii: S0167-7012(16)30021-5. doi: 10.1016/j.mimet.2016.02.004. [Epub ahead of print]
26851674
Implications of human TRPM8 channel gating from menthol binding studies of the sensing domain.
Author : Rath P, Hilton JK, Sisco NJ, Van Horn WD.
Biochemistry. 2015 Dec 11. [Epub ahead of print]
26653082
131I therapy mediated by sodium/iodide symporter combined with kringle 5 has a synergistic therapeutic effect on glioma.
Author : Shi S1, Zhang M1, Guo R1, Zhang M1, Hu J1, Xi Y1, Miao Y1, Li B1.
Oncol Rep. 2015 Nov 13. doi: 10.3892/or.2015.4420. [Epub ahead of print]
26572557
Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration-proliferation dichotomy.
Author : Bhandari D1, Lopez-Sanchez I2, To A3, Lo IC3, Aznar N2, Leyme A4, Gupta V3, Niesman I3, Maddox AL5, Garcia-Marcos M4, Farquhar MG6, Gh
Proc Natl Acad Sci U S A. 2015 Sep 1;112(35):E4874-83. doi: 10.1073/pnas.1514157112. Epub 2015 Aug 18.
26286990
TRAP150 interacts with the RNA-binding domain of PSF and antagonizes splicing of numerous PSF-target genes in T cells.
Author : Yarosh CA1, Tapescu I1, Thompson MG1, Qiu J2, Mallory MJ1, Fu XD2, Lynch KW3.
Nucleic Acids Res. 2015 Aug 10. pii: gkv816. [Epub ahead of print]
26261210
Venom allergen 5 is Associated With Deltamethrin Resistance in Culex pipiens pallens (Diptera: Culicidae).
Author : Lv Y1, Lei Z1, Hong S1, Wang W1, Zhang D1, Zhou D1, Sun Y1, Ma L1, Shen B1, Zhu C2.
J Med Entomol. 2015 Jul;52(4):672-82. doi: 10.1093/jme/tjv059. Epub 2015 May 29.
26335474
Efficient soluble expression of active recombinant human cyclin A2 mediated by E. coli molecular chaperones.
Author : Grigoroudis AI1, McInnes C2, Premnath PN2, Kontopidis G3.
Protein Expr Purif. 2015 May 6. pii: S1046-5928(15)00069-8. doi: 10.1016/j.pep.2015.01.013. [Epub ahead of print]
25956535
Region-Specific Activation of oskar mRNA Translation by Inhibition of Bruno-Mediated Repression.
Author : Kim G1, Pai CI1, Sato K2, Person MD3, Nakamura A4, Macdonald PM1.
PLoS Genet. 2015 Feb 27;11(2):e1004992. doi: 10.1371/journal.pgen.1004992. eCollection 2015.
25723530
Novel mechanism of hemin capture by Hbp2, the hemoglobin-binding hemophore from Listeria monocytogenes.
Author : Malmirchegini GR1, Sjodt M1, Shnitkind S1, Sawaya MR2, Rosinski J1, Newton SM3, Klebba PE3, Clubb RT4.
J Biol Chem. 2014 Dec 12;289(50):34886-99. doi: 10.1074/jbc.M114.583013. Epub 2014 Oct 14.
25315777
The disulfide bonding system suppresses CsgD-independent cellulose production in Escherichia coli.
Author : Hufnagel DA1, DePas WH2, Chapman MR3.
J Bacteriol. 2014 Nov;196(21):3690-9. doi: 10.1128/JB.02019-14. Epub 2014 Aug 11.
25112475
Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p.
Author : Liu D1, Novick P2.
J Cell Biol. 2014 Oct 13;207(1):59-72. doi: 10.1083/jcb.201404122.
25313406
Development of a functional antibody by using a green fluorescent protein frame as the template.
Author : Wang R1, Xiang S1, Zhang Y1, Chen Q1, Zhong Y1, Wang S2.
Appl Environ Microbiol. 2014 Jul;80(14):4126-37. doi: 10.1128/AEM.00936-14. Epub 2014 May 2.
24795367
Synthesis of phenoxyacyl-ethanolamides and their effects on fatty acid amide hydrolase activity.
Author : Faure L1, Nagarajan S, Hwang H, Montgomery CL, Khan BR, John G, Koulen P, Blancaflor EB, Chapman KD.
J Biol Chem. 2014 Mar 28;289(13):9340-51. doi: 10.1074/jbc.M113.533315. Epub 2014 Feb 20.
24558037
Characterization and expression of a GDSL-like lipase gene from Brassica napus in Nicotiana benthamiana.
Author : Tan X1, Yan S, Tan R, Zhang Z, Wang Z, Chen J.
Protein J. 2014 Feb;33(1):18-23. doi: 10.1007/s10930-013-9532-z.
24363150
Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp.
Author : Wang R1, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin M, Wang S.
Front Cell Infect Microbiol. 2013 Nov 6;3:72. doi: 10.3389/fcimb.2013.00072. eCollection 2013.
24224158
The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p.
Author : Shen D1, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, Reinisch K, McNew JA, Novick P.
J Cell Biol. 2013 Aug 5;202(3):509-26. doi: 10.1083/jcb.201211148. Epub 2013 Jul 29.
23897890
Methylation of the PTPRO gene in human hepatocellular carcinoma and identification of VCP as its substrate.
Author : Hsu SH, Motiwala T, Roy S, Claus R, Mustafa M, Plass C, Freitas MA, Ghoshal K, Jacob ST.
J Cell Biochem. 2013 Aug;114(8):1810-8. doi: 10.1002/jcb.24525.
23533167
Semiquinone and cluster N6 signals in His-tagged proton-translocating NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli.
Author : Narayanan M1, Gabrieli DJ, Leung SA, Elguindy MM, Glaser CA, Saju N, Sinha SC, Nakamaru-Ogiso E.
J Biol Chem. 2013 May 17;288(20):14310-9. doi: 10.1074/jbc.M113.467803. Epub 2013 Mar 29.
23543743
Protein export by the mycobacterial SecA2 system is determined by the preprotein mature domain.
Author : Feltcher ME1, Gibbons HS, Ligon LS, Braunstein M.
J Bacteriol. 2013 Feb;195(4):672-81. doi: 10.1128/JB.02032-12. Epub 2012 Nov 30.
23204463
Recombinant Bacillus subtilis that grows on untreated plant biomass.
Author : Anderson TD1, Miller JI, Fierobe HP, Clubb RT.
Appl Environ Microbiol. 2013 Feb;79(3):867-76. doi: 10.1128/AEM.02433-12. Epub 2012 Nov 26.
23183968
Biochemical analysis of the canonical model for the mammalian circadian clock.
Author : Ye R, Selby CP, Ozturk N, Annayev Y, Sancar A.
J Biol Chem. 2011 Jul 22;286(29):25891-902. doi: 10.1074/jbc.M111.254680. Epub 2011 May 25.
21613214
Analysis of androgen receptor SUMOylation.
Author : Rytinki MM, Kaikkonen S, Sutinen P, Palvimo JJ.
Methods Mol Biol. 2011;776:183-97. doi: 10.1007/978-1-61779-243-4_12.
21796528
Multiple ATR-Chk1 pathway proteins preferentially associate with checkpoint-inducing DNA substrates.
Author : Yilmaz S, Sancar A, Kemp MG.
PLoS One. 2011;6(7):e22986. doi: 10.1371/journal.pone.0022986. Epub 2011 Jul 29.
21829571
Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin.
Author : Terasawa M, Toya M, Motegi F, Mana M, Nakamura K, Sugimoto A.
Genes Cells. 2010 Nov;15(11):1145-57. doi: 10.1111/j.1365-2443.2010.01451.x. Epub 2010 Oct 21.
20964796
Reconstitution of RPA-covered single-stranded DNA-activated ATR-Chk1 signaling.
Author : Choi JH1, Lindsey-Boltz LA, Kemp M, Mason AC, Wold MS, Sancar A.
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13660-5. doi: 10.1073/pnas.1007856107. Epub 2010 Jun 28.
20616048
Expression of two Listeria monocytogenes antigens (P60 and LLO) in Lactococcus lactis and examination for use as live vaccine vectors.
Author : Bahey-El-Din M, Casey PG, Griffin BT, Gahan CG.
J Med Microbiol. 2010 Aug;59(Pt 8):904-12. doi: 10.1099/jmm.0.018770-0. Epub 2010 May 20.
20488938
Tipin-replication protein A interaction mediates Chk1 phosphorylation by ATR in response to genotoxic stress.
Author : Kemp MG, Akan Z, Yilmaz S, Grillo M, Smith-Roe SL, Kang TH, Cordeiro-Stone M, Kaufmann WK, Abraham RT, Sancar A, Unsal-Kaçmaz K.
J Biol Chem. 2010 May 28;285(22):16562-71. doi: 10.1074/jbc.M110.110304. Epub 2010 Mar 15.
20233725
The extracellular matrix glycoprotein elastin microfibril interface located protein 2: a dual role in the tumor microenvironment.
Author : Mongiat M, Marastoni S, Ligresti G, Lorenzon E, Schiappacassi M, Perris R, Frustaci S, Colombatti A.
Neoplasia. 2010 Apr;12(4):294-304.
20360940
Allosteric gating of Son of sevenless activity by the histone domain.
Author : Yadav KK, Bar-Sagi D.
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3436-40. doi: 10.1073/pnas.0914315107. Epub 2010 Feb 4.
20133694
The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement.
Author : Unsal-Kaçmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, Sancar A, Kaufmann WK.
Mol Cell Biol. 2007 Apr;27(8):3131-42. Epub 2007 Feb 12.
17296725
Role of structural plasticity in signal transduction by the cryptochrome blue-light photoreceptor.
Author : Partch CL, Clarkson MW, Ozgür S, Lee AL, Sancar A.
Biochemistry. 2005 Mar 15;44(10):3795-805.
15751956

BACKGROUND

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

REFERENCES

Hochuli E, Doebeli H, and Schacher A. New metal chelate absorbent selective for proteins and peptides containing neighboring histidine residues. J. Chromatogr. 1987;411:177-184.

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