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GFP Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

Immunofluorescent analysis of GFP using either natural fluorescence (green) or an GFP antibody (red) in Hela(human cervical epithelial adenocarcinoma cell line) cells transfected with GFP recombinant protein. Formalin-fixed cells were permeabilized with 0. 1% Triton X-100 for 10 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were probed with an GFP monoclonal antibody (Product # AM1009a) at a dilution of 1:25 for 1 hour at 37C, and incubated with DyLight 555 goat anti-mouse IgG secondary antibody (Product # 1511348) at a dilution of 1:200 for 60 minutes at 37℃. The nuclear counter stain is DAPI (blue).
Anti-GFP Tag Antibody at 1:2000 dilution + GFP protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 28 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情
文献引用 : 22
实验流程
背景知识

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, E
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone Names	168AT1211
Calculated MW	27 kDa KDa

Additional info
Target/Specificity	Purified His-tagged GFP protein was used to produced this monoclonal antibody.
Dilution	IF~~1:25 WB~~1:2000
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GFP Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

Potato Mop-Top Virus Co-Opts the Stress Sensor HIPP26 for Long-Distance Movement.
Author : Cowan GH1,Roberts AG1,Jones S1,Kumar P2,Kalyandurg PB3,Gil JF3,Savenkov EI3,Hemsley PA1,4,Torrance L5,2. Plant Physiol. 2018 Mar;176(3):2052-2070. doi: 10.1104/pp.17.01698. Epub 2018 Jan 26.	29374107

Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity.
Author : Zhao Q1,Hu ZY1,Zhang JP2,Jiang JD3,Ma YY1,Li JR1,Peng ZG1,Chen JH1. Sci Rep. 2017 Sep 12;7(1):11250. doi: 10.1038/s41598-017-11105-3.	28900156

The common parasite Toxoplasma gondii induces prostatic inflammation and microglandular hyperplasia in a mouse model.
Author : Colinot DL1,Garbuz T1,2,Bosland MC3,Wang L1,Rice SE4,Sullivan WJ Jr1,2,Arrizabalaga G1,2,Jerde TJ1,4,5. Prostate. 2017 May 12. doi: 10.1002/pros.23362. [Epub ahead of print]	28497488

Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers.
Author : Arumughan A¹, Roske Y¹, Barth C¹, Forero LL¹, Bravo-Rodriguez K², Redel A¹, Kostova S¹, McShane E¹, Opitz R¹, Faelber K¹, Rau K¹, Miel Nat Commun. 2016 Oct 20;7:13047. doi: 10.1038/ncomms13047.	27762274

Inactivation of TGFβ receptors in stem cells drives cutaneous squamous cell carcinoma.
Author : Cammareri P¹, Rose AM², Vincent DF¹, Wang J³, Nagano A³, Libertini S¹, Ridgway RA¹, Athineos D¹, Coates PJ⁴, McHugh A², Pourreyron C², Nat Commun. 2016 Aug 25;7:12493. doi: 10.1038/ncomms12493.	27558455

Stress Granules Modulate SYK to Cause Microglial Cell Dysfunction in Alzheimer\'s Disease.
Author : Ghosh S¹, Geahlen RL¹. EBioMedicine. 2015 Oct 3;2(11):1785-98. doi: 10.1016/j.ebiom.2015.09.053. eCollection 2015.	26870803

Expression and Purification of the Alpha Subunit of the Epithelial Sodium Channel, ENaC.
Author : Reddy BG¹, Dai Q², McNicholas CM³, Fuller CM⁴, Kappes JC⁵, DeLucas LJ⁶. Protein Expr Purif. 2015 Sep 19. pii: S1046-5928(15)30064-4. doi: 10.1016/j.pep.2015.09.014. [Epub ahead of print]	26394093

Physical and Functional Interactions between a Glioma Cation Channel and Integrin β1 Require α-Actinin.
Author : Rooj AK¹, Liu Z², McNicholas CM², Fuller CM³. Am J Physiol Cell Physiol. 2015 Jun 24:ajpcell.00036.2015. doi: 10.1152/ajpcell.00036.2015. [Epub ahead of print]	26108662

Moesin and myosin phosphatase confine neutrophil orientation in a chemotactic gradient.
Author : Liu X¹, Yang T², Suzuki K³, Tsukita S³, Ishii M³, Zhou S², Wang G¹, Cao L¹, Qian F¹, Taylor S¹, Oh MJ¹, Levitan I¹, Ye RD J Exp Med. 2015 Feb 9;212(2):267-80. doi: 10.1084/jem.20140508. Epub 2015 Jan 19.	25601651

Best practices for fluorescence microscopy of the cyanobacterial circadian clock.
Author : Cohen SE¹, Erb ML², Pogliano J², Golden SS³. Methods Enzymol. 2015;551:211-21. doi: 10.1016/bs.mie.2014.10.014. Epub 2014 Dec 26.	25662459

The Toxoplasma gondii centrosome is the platform for internal daughter budding as revealed by a Nek1 kinase mutant.
Author : Chen CT¹, Gubbels MJ. J Cell Sci. 2013 Aug 1;126(Pt 15):3344-55. doi: 10.1242/jcs.123364. Epub 2013 May 31.	23729737

RpL22e, but not RpL22e-like-PA, is SUMOylated and localizes to the nucleoplasm of Drosophila meiotic spermatocytes.
Author : Kearse MG, Ireland JA, Prem SM, Chen AS, Ware VC. Nucleus. 2013 May-Jun;4(3):241-58. doi: 10.4161/nucl.25261. Epub 2013 Jun 6.	23778934

Low temperature and chemical rescue affect molecular proximity of DeltaF508-cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC).
Author : Qadri YJ¹, Cormet-Boyaka E, Rooj AK, Lee W, Parpura V, Fuller CM, Berdiev BK. J Biol Chem. 2012 May 11;287(20):16781-90. doi: 10.1074/jbc.M111.332031. Epub 2012 Mar 22.	22442149

Glioma-specific cation conductance regulates migration and cell cycle progression.
Author : Rooj AK, McNicholas CM, Bartoszewski R, Bebok Z, Benos DJ, Fuller CM. J Biol Chem. 2012 Feb 3;287(6):4053-65. doi: 10.1074/jbc.M111.311688. Epub 2011 Nov 30.	22130665

Interaction of ASIC1 and ENaC subunits in human glioma cells and rat astrocytes.
Author : Kapoor N, Lee W, Clark E, Bartoszewski R, McNicholas CM, Latham CB, Bebok Z, Parpura V, Fuller CM, Palmer CA, Benos DJ. Am J Physiol Cell Physiol. 2011 Jun;300(6):C1246-59. doi: 10.1152/ajpcell.00199.2010. Epub 2011 Feb 23.	21346156

Enhanced erythropoiesis in Hfe-KO mice indicates a role for Hfe in the modulation of erythroid iron homeostasis.
Author : Ramos P¹, Guy E, Chen N, Proenca CC, Gardenghi S, Casu C, Follenzi A, Van Rooijen N, Grady RW, de Sousa M, Rivella S. Blood. 2011 Jan 27;117(4):1379-89. doi: 10.1182/blood-2010-09-307462. Epub 2010 Nov 8.	21059897

Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase.
Author : Clark EB, Jovov B, Rooj AK, Fuller CM, Benos DJ. J Biol Chem. 2010 Aug 27;285(35):27130-43. doi: 10.1074/jbc.M110.153213. Epub 2010 Jul 2.	20601429

A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis.
Author : Lorestani A, Sheiner L, Yang K, Robertson SD, Sahoo N, Brooks CF, Ferguson DJ, Striepen B, Gubbels MJ. PLoS One. 2010 Aug 19;5(8):e12302. doi: 10.1371/journal.pone.0012302.	20808817

The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice.
Author : Maier B, Ogihara T, Trace AP, Tersey SA, Robbins RD, Chakrabarti SK, Nunemaker CS, Stull ND, Taylor CA, Thompson JE, Dondero RS, Lewis EC, Dinarello CA, Nadler JL, Mirmira RG. J Clin Invest. 2010 Jun;120(6):2156-70. doi: 10.1172/JCI38924. Epub 2010 May 24.	20501948

Knockdown of ASIC1 and epithelial sodium channel subunits inhibits glioblastoma whole cell current and cell migration.
Author : Kapoor N, Bartoszewski R, Qadri YJ, Bebok Z, Bubien JK, Fuller CM, Benos DJ. J Biol Chem. 2009 Sep 4;284(36):24526-41. doi: 10.1074/jbc.M109.037390. Epub 2009 Jun 26.	19561078

Kv4 accessory protein DPPX (DPP6) is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons.
Author : Kim J, Nadal MS, Clemens AM, Baron M, Jung SC, Misumi Y, Rudy B, Hoffman DA. J Neurophysiol. 2008 Oct;100(4):1835-47. doi: 10.1152/jn.90261.2008. Epub 2008 Jul 30.	18667548

Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus.
Author : Witola WH, Pessi G, El Bissati K, Reynolds JM, Mamoun CB. J Biol Chem. 2006 Jul 28;281(30):21305-11. Epub 2006 May 16.	16704982

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

REFERENCES

Ward, W. W., et al.(1980) Photochem. Photobiol. 31:611

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Cat# AM1009a

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GFP Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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