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>   首页   >   产品   >   一抗   >   精选抗体   >   标签抗体   >   HA Tag Antibody   

HA Tag Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - HA Tag Antibody AM1008a
    HA-tagged recombinant protein was expressed in COS7 cells. 15 μg of cell lysate was loaded onto a gel to test the HA tag antibody (AM1008a).
  • 1 - HA Tag Antibody AM1008a
    Western blot analysis of anti-HA Tag Antibody (AM1008a) in 12tag recombinant protein. 12tag recombinant protein was detected using the purified Mab.
  • 产品详情
  • 文献引用 : 23
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Names 12CA5
Calculated MW Da Da
Additional info
Other Names Tag from influenza hemagglutinin protein
Target/Specificity KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
Dilution WB~~1:1,000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Aquaporin-4 Cell-Surface Expression and Turnover Are Regulated by Dystroglycan, Dynamin, and the Extracellular Matrix in Astrocytes.
Author : Tham DK1, Joshi B1, Moukhles H1.
PLoS One. 2016 Oct 27;11(10):e0165439. doi: 10.1371/journal.pone.0165439. eCollection 2016.
27788222
Sp1 transcription factor promotes TMEPAI gene expression and contributes to cell proliferation.
Author : Li Y1, Guo A1, Feng Y1, Zhang Y1, Wang J1, Jing L1, Yan Y1, Jing L1, Liu Z1, Ma L1, Diao A2.
Cell Prolif. 2016 Sep 14. doi: 10.1111/cpr.12292. [Epub ahead of print]
27625141
DELLA proteins interact with FLC to repress flowering transition.
Author : Li M1,An F1,Li W1,Ma M1,Feng Y1,Zhang X1,Guo H1.
J Integr Plant Biol. 2016 Jul;58(7):642-55. doi: 10.1111/jipb.12451. Epub 2016 Mar 2.
26584710
Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis.
Author : Schmidt M1, Goebeler M2, Martin SF3.
Methods Mol Biol. 2016;1390:319-40. doi: 10.1007/978-1-4939-3335-8_20.
26803638
Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2.
Author : Drechsler H1, Tan AN1, Liakopoulos D2.
J Cell Sci. 2015 Nov 1;128(21):3910-21. doi: 10.1242/jcs.166686. Epub 2015 Sep 22.
26395399
Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP.
Author : Mizutani N1, Inoue M1, Omori Y1, Ito H1, Tamiya-Koizumi K1, Takagi A1, Kojima T1, Nakamura M2, Iwaki S3, Nakatochi M4, Suzuki M5, Noza
J Biochem. 2015 Oct;158(4):309-19. doi: 10.1093/jb/mvv039. Epub 2015 Apr 17.
25888580
Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth.
Author : Mertz J1, Tan H2, Pagala V2, Bai B1, Chen PC1, Li Y1, Cho JH2, Shaw T3, Wang X2, Peng J4.
Mol Cell Proteomics. 2015 Jul;14(7):1898-910. doi: 10.1074/mcp.M114.045898. Epub 2015 Apr 30.
25931508
Maintaining glycogen synthase kinase-3 activity is critical for mTOR kinase inhibitors to inhibit cancer cell growth.
Author : Koo J1, Yue P, Gal AA, Khuri FR, Sun SY.
Cancer Res. 2014 May 1;74(9):2555-68. doi: 10.1158/0008-5472.CAN-13-2946. Epub 2014 Mar 13.
24626091
Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells.
Author : Bruntz RC1, Taylor HE, Lindsley CW, Brown HA.
J Biol Chem. 2014 Jan 10;289(2):600-16. doi: 10.1074/jbc.M113.532978. Epub 2013 Nov 20.
24257753
The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition.
Author : Li B1, Ren H, Yue P, Chen M, Khuri FR, Sun SY.
Cancer Prev Res (Phila). 2012 Apr;5(4):612-20. doi: 10.1158/1940-6207.CAPR-11-0548. Epub 2012 Feb 16.
22345097
HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation.
Author : Sun XD, Ni M.
Mol Plant. 2011 Jan;4(1):116-26. doi: 10.1093/mp/ssq055. Epub 2010 Sep 23.
20864454
Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation.
Author : Satow R1, Shitashige M, Jigami T, Honda K, Ono M, Hirohashi S, Yamada T.
J Biol Chem. 2010 Aug 20;285(34):26289-94. doi: 10.1074/jbc.M109.090597. Epub 2010 Jun 21.
20566648
Angiotensin II stimulates thick ascending limb superoxide production via protein kinase C(α)-dependent NADPH oxidase activation.
Author : Herrera M, Silva GB, Garvin JL.
J Biol Chem. 2010 Jul 9;285(28):21323-8. doi: 10.1074/jbc.M110.109157. Epub 2010 May 6.
20448043
c-FLIP degradation mediates sensitization of pancreatic cancer cells to TRAIL-induced apoptosis by the histone deacetylase inhibitor LBH589.
Author : Kauh J, Fan S, Xia M, Yue P, Yang L, Khuri FR, Sun SY.
PLoS One. 2010 Apr 28;5(4):e10376. doi: 10.1371/journal.pone.0010376.
20442774
FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms.
Author : Czymai T, Viemann D, Sticht C, Molema G, Goebeler M, Schmidt M.
J Biol Chem. 2010 Apr 2;285(14):10163-78. doi: 10.1074/jbc.M109.056663. Epub 2010 Feb 1.
20123982
Blimp1-mediated repression of negative regulators is required for osteoclast differentiation.
Author : Nishikawa K, Nakashima T, Hayashi M, Fukunaga T, Kato S, Kodama T, Takahashi S, Calame K, Takayanagi H.
Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3117-22. doi: 10.1073/pnas.0912779107. Epub 2010 Jan 28.
20133620
Beta-catenin/T-cell factor signaling is activated during lung injury and promotes the survival and migration of alveolar epithelial cells.
Author : Flozak AS, Lam AP, Russell S, Jain M, Peled ON, Sheppard KA, Beri R, Mutlu GM, Budinger GR, Gottardi CJ.
J Biol Chem. 2010 Jan 29;285(5):3157-67. doi: 10.1074/jbc.M109.070326. Epub 2009 Nov 20.
19933277
Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis.
Author : Guo M, Thomas J, Collins G, Timmermans MC.
Plant Cell. 2008 Jan;20(1):48-58. doi: 10.1105/tpc.107.056127. Epub 2008 Jan 18.
18203921
CCAAT/enhancer binding protein homologous protein-dependent death receptor 5 induction and ubiquitin/proteasome-mediated cellular FLICE-inhibitory protein down-regulation contribute to enhancement of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by dimethyl-celecoxib in human non small-cell lung cancer cells.
Author : Chen S, Liu X, Yue P, Schönthal AH, Khuri FR, Sun SY.
Mol Pharmacol. 2007 Nov;72(5):1269-79. Epub 2007 Aug 7.
17684158
Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1.
Author : Choe EA, Liao L, Zhou JY, Cheng D, Duong DM, Jin P, Tsai LH, Peng J.
J Neurosci. 2007 Aug 29;27(35):9503-12.
17728463
Phenethyl isothiocyanate, a cancer chemopreventive constituent of cruciferous vegetables, inhibits cap-dependent translation by regulating the level and phosphorylation of 4E-BP1.
Author : Hu J, Straub J, Xiao D, Singh SV, Yang HS, Sonenberg N, Vatsyayan J.
Cancer Res. 2007 Apr 15;67(8):3569-73.
17440067
Genetic regulators of large-scale transcriptional signatures in cancer.
Author : Adler AS, Lin M, Horlings H, Nuyten DS, van de Vijver MJ, Chang HY.
Nat Genet. 2006 Apr;38(4):421-30. Epub 2006 Mar 5.
16518402
RACK1 regulates G1/S progression by suppressing Src kinase activity.
Author : Mamidipudi V, Zhang J, Lee KC, Cartwright CA.
Mol Cell Biol. 2004 Aug;24(15):6788-98.
15254245

BACKGROUND

Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.

REFERENCES

Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.

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